Gut Microbes Ease Constipation

“Probiotic strains were often effective in animal models yet failed in human clinical trials or were poorly validated in humans,” says Jiachao Zhang of Hainan University, the study’s second co-senior author. “Proof-of-concept studies based on a human cohort in combination with evidence from animal studies are urgently needed for translational research.”

Zhai, Zhang, and Shi Huang of the University of Hong Kong, the paper’s third co-senior author, set out to identify and systematically validate the key genetic factors of exogenous probiotics or resident gut microbiota affecting gastrointestinal motility. They isolated 185 B. longum strains from 354 Chinese subjects who ranged in age from 0 to 108 years.

This key genetic factor preferentially enhances the utilization of arabinan—a common constituent of plant polysaccharides, an indigestible fiber for humans, and a poorly accessible source of nutrients for normal gut microbes.

The researchers further validated the abfA cluster’s functional roles using gene-knockout experiments. In mice with constipation, B. longum, but not an abfA mutant, improved gastrointestinal transit time—an effect that was dependent upon dietary arabinan. <

Probing Functional Roles in Alleviating Constipation

To establish its functional roles for ameliorating constipation in humans, the researchers used a clinical trial and a human-to-mouse fecal microbiota transplantation experiment in combination with metagenomics and metabolomics. In the double-blind, randomized, placebo-controlled clinical trial, supplementation with abfA-cluster-carrying B. longum, but not an abfA-deficient strain, enriched arabinan-utilization residents, increased beneficial metabolites, and improved constipation symptoms.

Across human cohorts, abfA-cluster abundance in the fecal microbiomes predicted constipation, and transplantation of abfA cluster-enriched human microbiota to mice with constipation improved gut motility. Notably, other than B. longum, the abfA gene/cluster is prevalent in gut residents, regulating symptoms in both mice and humans.

The authors say that the abfA cluster is a gut-microbiome therapeutic target for constipation in humans. More broadly, the results suggest that genetic factors governing the unique metabolic capability of probiotics should be primarily considered for screening probiotics or inferring their treatment efficacy for gastrointestinal diseases.

“Collectively, this study identified and systematically characterized a key genetic factor responsible for arabinan utilization that addressed one critical challenge in the probiotic field, namely widespread yet unknown strain specificity in probiotic treatment efficacy,” Huang says. “Our proof-of-concept study also established generalizable principles for the rational development of colonizable, functional probiotics with persistent treatment efficacy in multiple model organisms.”

Source: Eurekalert